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Purposeful validation of sorted fractions was done by xenotransplantation, the result of which was coupled with miRNA expression profiling to generate stem mobile-relevant miRNA expression profiles.
To ascertain regardless of whether miRNA are differentially expressed in LSC and HSC, we fractionated sixteen AML individual samples and a few lineage-depleted (Lin–) cord blood (CB) samples applying CD34 and CD38 into four populations and subjected Each individual to world-wide miRNA profiling; the stem mobile written content of each fraction was functionally assayed by xenotransplantation (Figures 1A and S1A).
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(J) Proliferation measured by BrdU incorporation assay of CTRL or 126KD transduced 8227 cells in vitro.
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The LSC frequency was lowered in two of a few samples on 126KD (Figures 5C and S5D). Jointly, these conclusions propose that 126KD generates heterogeneous responses with LSC function and frequency decreased in a subset of AML patients.
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(E) Percent BrdU incorporation into bulk cultures showing proliferation of CTRL and 126OE transduced 8227 cells over time.
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